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Idiopathic Intracranial Hypertension: The Brain Glymphoedema

The recent discoveries of the glymphatic system and of dural lymphatic vessels represent major breakthroughs in basic neurosciences, allowing new insights into complex neurological conditions.

Stephanie Lenck is the first physician researcher to have understood the role of the venolymphatic system in IIH. In this article written for IIH-Hub, she summarizes her findings, first published in 2018 in the Journal Neurology (reference 6). Dr. Lenck will be presenting on IIH and Brain Glymphedema on May 17 at 9 am ET as part of the IIH Practitioner Series – click here to learn more and register for the live lecture. 


The recent discoveries of the glymphatic system and of dural lymphatic vessels represent major breakthroughs in basic neurosciences, allowing new insights into complex neurological conditions1-5. In light of these findings, the interplay between the circulation of CSF and the cerebrovascular system (including arteries, veins and lymphatics) seems to be more intimately related than previously suspected.  In the broader context of these basic science findings, idiopathic intracranial hypertension (IIH) has been recently summarized in the following pathological triad: “restriction of the venous CSF outflow pathway – overflow of the lymphatic CSF outflow pathway – congestion of the glymphatic system”6, 7. Lateral sinus stenoses (LSS) may thus represent the macroscopic evidence of the microscopic restriction in the venous CSF outflow pathway in IIH. These stenoses lead to an increase in the cerebral venous pressure, thereby interrupting the passive resorption of CSF from the subarachnoid space to the venous blood of the dural sinuses. These stenoses thus seem to play a crucial role in maintaining and/or triggering the ‘vicious cycle’ of IIH. By restoring a physiological pressure gradient between the venous system and the subarachnoid space, venous sinus stenting (VSS) allows us to break this cycle and in many cases effectively relieve the symptoms related to elevated intracranial pressure (ICP). However, IIH symptoms may recur in about 10 % of patients8. In most of these relapsed cases, a stenosis of the stent-adjacent ipsilateral transverse sinus and/or of the proximal third of the superior longitudinal sinus (SLS) is found, which seems to re-trigger the pathological loop of IIH9.

Techniques of exploration of the venous sinuses and parasinuses in humans and mice    

A: schematic representation of the veno-lymphatic system in humans. Blue: dural venous sinuses, yellow: dural lymphatic system

B: 3D reconstruction of the veno-lymphatic system using MRI acquisition in humans according to the technique described in Jacob et al. Jexp Med 2022

C and D: Exploration of the parasinus in mice using immunofluorescence labeling on whole mounts preparations (courtesy to Marie-Renée El Kamouh, Paris Brain Institute)



The Glymphatic System

In 2012, Iliff et al. identified the glymphatic system as “a brain-wide pathway for fluid transport, which includes the para-arterial influx of subarachnoid CSF into the brain interstitium, followed by the clearance of interstitial fluid along large-caliber draining veins”3. This process is preferentially activated during sleep10, and is driven by a combination of arterial pulsatility, respiration, and  pressure gradients4, 11. The exchange of water molecules between the three compartments of the brain (i.e. the blood, the CSF and the brain parenchyma) is mediated by water-channel transporters called Aquaporins (AQP). CSF is continuously produced by the choroid plexuses  through osmotic- and pressure-gradients that drive the movement of water and ions from the blood to the ventricular lumen10. From the ventricles, the CSF then exits through the foramina of Magendie and Monro to reach the subarachnoid space10. From the subarachnoid spaces, CSF then enters the periarterial spaces, travelling from the cortex toward the deep white matter along the courses of the pial and perforator arteries10. Along with other metabolites, CSF is then filtered and driven from the periarterial space to the brain parenchyma10. The transport of water from CSF to the brain is mediated by another water-transporter, AQP-43. This continuous movement of CSF from the periarterial space into the brain parenchyma then drives convective bulk parenchymal fluid flow toward the perivenous spaces surrounding the large cortical veins3. Following this, the method of resorption of CSF from the perivenous spaces is still unclear. Two CSF outflow pathways have been described in humans: the venous outflow pathway and the lymphatic outflow pathway.

CSF outflows

While the venous CSF outflow pathway has historically been considered as the only way of resorption of CSF, the recent discovery of the dural lymphatics in humans has been another major paradigm shift12. To better describe the CSF outflow pathways, we first need to clearly distinguish two physiological roles of CSF: a mechanical role which plays a role in the regulation of ICP, and a metabolic one which plays a role in clearance of brain metabolites.

The venous CSF outflow pathway

It was historically believed that the venous resorption of the CSF occurs across the arachnoid villi and granulations. These anatomical structures are traditionally described as focal areas of protrusion of the subarachnoid space across the dura matter into the lumen of the dural sinuses. These “avascular granulations” also play a mechanical role of regulation of the ICP, as the flow of CSF across the granulation is dependent on the pressure gradient between the subarachnoid space and the venous blood of the dural sinus. In the light of the recent scientific findings concerning the glymphatic system, it seems that another type of granulations – the so-called “vascular granulation” – has probably been wrongly neglected over time. Previous pathological13, 14 and radiological studies14, 15, support that some arachnoid granulations may enter the dura mater to reach the lumen of the venous sinuses in close association with a major cortical vein. These “vascular granulations” could represent an anatomical and physiological connection between the perivenous space (draining the ISF from the glymphatic system) and the venous blood of the dural sinus14. Vascular granulations may therefore be involved in the excretion of the brain metabolites as one final exit pathway of the glymphatic system. The intrinsic molecular mechanisms of this filtration are however still unknown.

The lymphatic CSF outflow pathway

It was long believed that the CNS did not have a lymphatic drainage system. Ironically, an Italian anatomist called Paola Mascagni described meningeal-related lymphatic vessels in a landmark anatomical text in 1787, but her findings were discounted by the scientific community for more than 200 years16. In 2015, the presence of functional lymphatic vessels lining the dural sinuses was eventually demonstrated in murine brains2, 5. Two years later, Absinta et al went on to image these dural/meningeal lymphatics in both primates and humans1. They also seem to be involved in the clearance of the CSF (or ISF) from the glymphatic system2, 17, and also in the regulation of the ICP (through a direct reabsorption of the CSF from the subarachnoid space)5. Other work by Hoon Ahn et al, showed that CSF drains preferentially through a basal outflow pathway, with CSF tracers draining via skull base meningeal lymphatics to the deep cervical lymph node system. Anatomically, the lymphatic system of the brain could therefore be described as a drainage network extending from the dural sinuses to both eyes, tracking above the olfactory bulb, following the dural arteries and veins into the dura matter2, 5. The dural lymphatics finally join the skull base, discharging the CSF into the sheaths of the cranial nerves. The CSF is eventually excreted into the deep cervical lymph nodes and the systemic lymphatic circulation18.

Several radiological studies indicate that IIH is associated with an increase in CSF in the perivascular spaces of the brain and in the subarachnoid space, suggesting a congestion of the glymphatic system.


In the light of these scientific findings, the radiological signs of IIH can be summarized in the following pathological triad (Fig. 1)6:

  1. Congestion of the glymphatic system
  2. Overflow of the lymphatic CSF outflow pathway
  3. Restriction of the venous CSF outflow pathway

IIH: congestion of the glymphatic system

Several radiological studies indicate that IIH is associated with an increase in CSF in the perivascular spaces of the brain and in the subarachnoid space, suggesting a congestion of the glymphatic system. Since the skull represents a fixed volume, the excess of CSF in the glymphatic system results in increased intracranial pressure. The first radiological observations of IIH were based on CT-scans and showed a reduction of ventricular size, suggesting that IIH was due to cerebral swelling19. This interstitial edema was confirmed later with MRI diffusion techniques and with 3D-volumetric MRI sequences20. Alperin et al. showed a significant increase in extra-ventricular CSF and interstitial fluid volumes in patients with IIH, when compared to a matched cohort of patients without IIH.

IIH: overflow of the lymphatic CSF outflow pathway

Imaging evidence of excess CSF along the sheaths of cranial nerves is one of the cardinal signs of IIH. Most typically this is found along the optic nerve sheaths, however the sheaths of other cranial nerves can also be enlarged. This may be a consequence of the accumulation of CSF along the sheaths of the cranial nerves. This excess of CSF appears to be related to the engorgement of the lymphatic CSF outflow pathway21, 22. For example, erosion of the cribriform plate (which may result in idiopathic CSF leak) may be the consequence of the chronic overflow of CSF around the olfactory bulbs23. Other cardinal imaging signs of IIH are also likely related to an excess of CSF along the relevant nerve sheaths.

Restriction of the venous CSF outflow pathway

More than 90% of patients with IIH have lateral sinus stenoses, which are usually located bilaterally at the junction between the vein of Labbé and the transverse sinus24. Those stenoses can result in increased cerebral venous pressure (CVP), leading in turn to a less efficient venous CSF outflow pathway as a result of equalization of the pressure gradient between the subarachnoid space and the venous blood of the dural sinuses. Although LSS  are probably the main precipitating factor in the occurrence of clinical symptoms in IIH – and the resolution of symptoms after venous stenting gives support to this hypothesis – the cause of these stenoses remains unclear. However, it is likely that a molecular impairment of CSF filtration at the venodural junction may be responsible for the formation of LSS, either through the formation of an arachnoid granulation and/or through a modification of the water content of the dura mater resulting in an hyper collapsibility of the wall of the dural sinuses25. We presume that the metabolic and hormonal factors associated with IIH (obesity, hormones, drugs…) may be involved in this molecular trigger.


Accordingly, it is crucial that we improve our knowledge of venous sinus stenoses. Although the dichotomization of stenoses into extrinsic and intrinsic stenoses appears to have some relevance to clinical practice and to neurointerventions, it is probably a crude approximation of the underlying morphology of the stenosis26. These observations should thus prompt us to keep exploring the underlying pathophysiological mechanisms of LSS and to improve our knowledge of the venous physiology and better understand how it relates to CSF circulation.

  1. Absinta M, Ha SK, Nair G, et al. Human and nonhuman primate meninges harbor lymphatic vessels that can be visualized noninvasively by MRI. Elife 2017;6.
  2. Aspelund A, Antila S, Proulx ST, et al. A dural lymphatic vascular system that drains brain interstitial fluid and macromolecules. J Exp Med 2015;212:991-999.
  3. Iliff JJ, Wang M, Liao Y, et al. A paravascular pathway facilitates CSF flow through the brain parenchyma and the clearance of interstitial solutes, including amyloid beta. Sci Transl Med 2012;4:147ra111.
  4. Jessen NA, Munk AS, Lundgaard I, Nedergaard M. The Glymphatic System: A Beginner’s Guide. Neurochem Res 2015;40:2583-2599.
  5. Louveau A, Smirnov I, Keyes TJ, et al. Structural and functional features of central nervous system lymphatic vessels. Nature 2015;523:337-341.
  6. Lenck S, Radovanovic I, Nicholson P, Hodaie M, Krings T, Mendes-Pereira V. Idiopathic intracranial hypertension: The veno glymphatic connections. Neurology 2018;91:515-522.
  7. Nicholson P, Kedra A, Shotar E, et al. Idiopathic Intracranial Hypertension: Glymphedema of the Brain. J Neuroophthalmol 2020.
  8. Nicholson P, Brinjikji W, Radovanovic I, et al. Venous sinus stenting for idiopathic intracranial hypertension: a systematic review and meta-analysis. J Neurointerv Surg 2019;11:380-385.
  9. Kumpe DA, Seinfeld J, Huang X, et al. Dural sinus stenting for idiopathic intracranial hypertension: factors associated with hemodynamic failure and management with extended stenting. J Neurointerv Surg 2017;9:867-874.
  10. Benveniste H, Lee H, Volkow ND. The Glymphatic Pathway. Neuroscientist 2017:1073858417691030.
  11. Iliff JJ, Wang M, Zeppenfeld DM, et al. Cerebral arterial pulsation drives paravascular CSF-interstitial fluid exchange in the murine brain. J Neurosci 2013;33:18190-18199.
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  18. Weller RO, Djuanda E, Yow HY, Carare RO. Lymphatic drainage of the brain and the pathophysiology of neurological disease. Acta Neuropathol 2009;117:1-14.
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  20. Alperin N, Ranganathan S, Bagci AM, et al. MRI evidence of impaired CSF homeostasis in obesity-associated idiopathic intracranial hypertension. AJNR Am J Neuroradiol 2013;34:29-34.
  21. Bidot S, Saindane AM, Peragallo JH, Bruce BB, Newman NJ, Biousse V. Brain Imaging in Idiopathic Intracranial Hypertension. J Neuroophthalmol 2015;35:400-411.
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  23. Perez MA, Bialer OY, Bruce BB, Newman NJ, Biousse V. Primary spontaneous cerebrospinal fluid leaks and idiopathic intracranial hypertension. J Neuroophthalmol 2013;33:330-337.
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  25. Ficek K, Kedra N, Skowronek R, et al. The Fifth Metatarsal Bone Fracture in Athletes – Modalities of Treatment Related to Agility in Soccer Players. J Hum Kinet 2021;79:101-110.
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